This Fiji macro allows a user to perform semi-automated quantification of 2D IHC colocalization and RNAscope puncta counting in fluorescent microscopy images. Put all the images you'd like to analyze ...
This Fiji macro allows a user to perform semi-automated quantification of 2D IHC colocalization and RNAscope puncta counting in fluorescent microscopy images. Put all the images you'd like to analyze ...
Single-Molecule Co-Localization Analysis原理ボロノイ図(Voronoi diagram)を簡単に説明する。平面上に、いくつかの点が配置されている。このとき、その平面内の点を、どの点に最も近いかによって分割してできる図のこと(下図)。定義としては、「隣り合う母点間を ...
Insight into the function and regulation of biological molecules can often be obtained by determining which cell structures and other molecules they localize with (i.e. colocalization). Here we ...
Fluorescence resonance energy transfer (FRET) between an adequate pair of fluorophores is an indication of closer proximity than colocalization and is used by biologists to study fluorescently ...
An increasing amount of biological and medical research relies on single-cell imaging to obtain information about the phenotypic response of cells to a variety of chemical, mechanical and genetic ...